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| Registros recuperados: 87 | |
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| Bellafronte,Elisangela; Mariguela,Tatiane Casagrande; Pereira,Luiz Henrique Garcia; Oliveira,Claudio; Moreira-Filho,Orlando. |
| In the past years, DNA barcoding has emerged as a quick, accurate and efficient tool to identify species. Considering the difficulty in identifying some Parodontidae species from the La Plata basin and the absence of molecular data for the group, we aimed to test the effectiveness of DNA barcoding and discuss the importance of using different approaches to solve taxonomic problems. Eight species were analyzed with partial sequences of Cytochrome c oxidase I. The mean intraspecific K2P genetic distance was 0.04% compared to 4.2% for mean interspecific K2P genetic distance. The analyses of distance showed two pairs of species with K2P genetic divergence lower than 2%, but enough to separate these species. Apareiodon sp. and A. ibitiensis, considered as the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Apareiodon; COI gene; Cytogenetics; Fish identification; Morphology. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252013000300497 |
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| Castro,Jonathan P.; Moura,Maurício O.; Moreira-Filho,Orlando; Shibatta,Oscar A.; Santos,Mateus H.; Nogaroto,Viviane; Vicari,Marcelo R.; Almeida,Mara C. de; Artoni,Roberto F.. |
| Two populations of the Astyanax scabripinniscomplex, isolated by a waterfall with over 100 meters depth and inhabiting different altitudes of the same river (1850 m a.s.l. and 662 m a.s.l.) were compared in reproductive data, geometric morphometry, tooth morphology, anal-fin rays counts, and karyotype, in order to test the hypothesis of speciation between the two populations. The results in the geometric morphometry analysis showed differences between the populations. Discriminant function analysis (DFA) and canonical variance analysis revealed sexual dimorphism. Secondary sexual characters, such as hooks in the anal fin rays of the males are absent in the lower altitude population. Both populations had the same macro karyotype structure, except for the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Allopatric populations; Cytogenetics; Evolution; Geometric morphometry; Paraíba do Sul River. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252014000200429 |
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| Zappelini,Julia; Souza,Luiz Gustavo; Guerra,Miguel Pedro; Pescador,Rosete. |
| ABSTRACT Although karyotype features are useful data for evolutionary studies, cytogenetic data in Bambusoideae are mainly based only on chromosome counts. The first comparative cytogenetic analysis of three Neotropical woody bamboo species - Guadua chacoensis, G. angustifolia and Chusquea tenella - was undertaken based on new and reviewed chromosome counts, CMA/DAPI double staining, fluorescent in situ hybridization (FISH) with 35S and 5S rDNA probes, and genome size estimation. The two species of Guadua were found to have 2n = 46 chromosomes, while the first record for C. tenella was 2n = 44. Only one pair of CMA+/DAPI- was detected on the terminal region of metacentric chromosomes in all three species. Likewise, one pair of 5S and 35S rDNA sites was... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Chusquea; Cytogenetics; Diploidization; Evolution; Guadua; Polyploidy. |
| Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062020000400673 |
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| Zanella,Camila M.; Janke,Aline; Palma-Silva,Clarisse; Kaltchuk-Santos,Eliane; Pinheiro,Felipe G.; Paggi,Gecele M.; Soares,Luis E.S.; Goetze,Márcia; Büttow,Miriam V.; Bered,Fernanda. |
| Bromeliaceae is a morphologically distinctive and ecologically diverse family originating in the New World. Three centers of diversity, 58 genera, and about 3,140 bromeliad species are currently recognized. We compiled all of the studies related to the reproductive biology, genetic diversity, and population structure of the Bromeliaceae, and discuss the evolution and conservation of this family. Bromeliads are preferentially pollinated by vertebrates and show marked variation in breeding systems, from predominant inbreeding to predominant outcrossing, as well as constancy in chromosome number (2n = 2x = 50). Autogamous or mixed mating system bromeliads have a high inbreeding coefficient (F IS), while outcrossing species show low F IS. The degree of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bromeliads; Cytogenetics; Genetic diversity; Population structure; Reproductive biology. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000600017 |
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| Katarzyna, S; Renault, Tristan; Wolowicz, M. |
| This study presents results of histopathologic, cytogenetics and flow cytometry analyses performed on Macoma balthica collected from the Gulf of Gdansk (Baltic Sea) in 2003 in order to compare the techniques for diagnosis of neoplasia. The proportion of affected clams gave a crude prevalence of 15.7 %. The four stages of the disease defined by histology and three stages of neoplasia defined by flow cytometry were reported. Stage I defined by flow cytometry corresponded to stages I and II defined by histology. Chromosome analysis did not lead to a staging of neoplastic progression. Both cytogenetics and flow cytometry indicated a difference in the DNA content of non-neoplastic and neoplastic cells. Cytogenetics examination marked that the range of... |
| Tipo: Text |
Palavras-chave: Baltic Sea; Cytofluorymetry; Cytogenetics; Histology; Macoma balthica; Neoplasia. |
| Ano: 2005 |
URL: http://archimer.ifremer.fr/doc/2005/publication-2921.pdf |
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| Bitencourt,Jamille de Araújo; Affonso,Paulo Roberto Antunes de Mello; Giuliano-Caetano,Lucia; Dias,Ana Lucia. |
| Few chromosomal reports are available for the endemic fish fauna from coastal basins in northeastern Brazil, and regional biodiversity remains partially or completely unknown. This is particularly true for Loricariidae, the most diverse family of armored catfishes. In the present work, allopatric populations of Hypostomus cf. wuchereri (Siluriformes: Loricariidae) from two basins in Bahia (northeastern Brazil) were cytogenetically analyzed. Both populations shared 2n = 76 chromosomes, a karyotype formula of 10m+18sm+48st/a (FN = 104) and single terminal GC-rich NORs on the second metacentric pair. Nevertheless, microstructural differences were detected by C-banding, fluorochrome staining and chromosomal digestion with restriction enzymes (Alu I, Bam HI,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Biodiversity; Cytogenetics; Heterochromatin; Hypostominii. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252011000200008 |
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| Pereira,Núbia P.; Ventura,Karen; Silva Júnior,Magno C.; Silva,Daniela de Melo e; Yonenaga-Yassuda,Yatiyo; Pellegrino,Katia C. M.. |
| The karyotypes of 23 specimens belonging to 16 species from nine genera of Brazilian marsupials (family Didelphidae) were studied. The animals were collected in eight localities of Cerrado or Atlantic Forest biomes in the states of Goiás, Tocantins and São Paulo. The karyotypes were analyzed after conventional Giemsa staining and silver staining of the nucleolus organizer regions (Ag-NORs). New karyotypic data were obtained for Gracilinanus microtarsus (2n = 14, FN = 24), Marmosops paulensis (2n = 14, FN = 24) , Micoreus paraguayanus (2n = 14, FN = 20) and Monodelphis rubida (2n = 18, FN = 32) and are discussed in detail. The karyotypes of G. microtarsus , M. paulensis and M. paraguayanus include three large pairs of submetacentrics (pairs 1, 2... |
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Palavras-chave: Marsupials; Karyotypes; Cytogenetics; Didelphidae; NORs. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000500014 |
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| Domingues,Alayne Magalhães Trindade; Waldschmidt,Ana Maria; Andrade,Sintia Emmanuelle; Andrade-Souza,Vanderly; Alves,Rogério Marco de Oliveira; Silva Junior,Juvenal Cordeiro da; Costa,Marco Antônio. |
| Although many species of the genus Trigona have been taxonomically described, cytogenetic studies of these species are still rare. The aim of the present study was to obtain cytogenetic data by conventional staining, C banding and fluorochrome staining for the karyotype characterization of the species Trigona fulviventris. Cytogenetic analysis revealed that this species possesses a diploid chromosome number of 2n = 32, different from most other species of this genus studied so far. This variation was probably due to the centric fusion in a higher numbered ancestral karyotype, this fusion producing the large metacentric chromosome pair and the lower chromosome number observed in Trigona fulviventris. Heterochromatin was detected in the pericentromeric... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Cytogenetics; Heterochromatin; Evolution; Stingless bees. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000300009 |
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| RAMOS,ROSEMAR S. L.; VALE,WILLIAM G.; ASSIS,FÁTIMA L.. |
| A total of 30 animals of the genus Dasyprocta were cytogenetically studied. They belong to the following species: D. prymnolopha (N=20), D. leporina (N=6), D. fuliginosa (N=1) and Dasyprocta sp. (N=3) (Dasyproctidae, Hystricognathi). Cell suspensions were obtained by peripheral blood culture, besides bone marrow and spleen cells, from D. prymnolopha and D. leporina. The diploid number was 64/65 for all samples. The karyotypes showed similarity, and chromosomal polymorphism was not detected by Giemsa conventional staining and G banding. The constitutive heterochromatin distribution at the pericentromeric region of all the chromosomes was similar in all species. D. prymnolopha, D. leporina and Dasyprocta sp. presented variation in the heterochromatical block... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cytogenetics; Hystricognathi; Dasyprocta; Karyotype. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652003000100007 |
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| Silva,Danillo S.; Peixoto,Luiz A. W.; Pieczarka,Julio C.; Wosiacki,Wolmar B.; Ready,Jonathan S.; Nagamachi,Cleusa Y.. |
| Eigenmannia species are widely distributed in the Neotropics, with eight valid species currently recognized. Populations of Eigenmannia from three locations in the eastern Amazon were investigated using cytogenetic and morphological techniques, revealing two taxa designated here as Eigenmannia sp. "A" and Eigenmannia sp. "B". The species differ in three morphometric characters, two meristic characters, and one osteological character. Eigenmannia sp. "A" presents 2n = 34 (22 m/sm+12 st/a) and Eigenmannia sp. "B" presents 2n = 38 (14 m/sm+24st/a) and simple differentiated sex chromosomes of the type XX/XY. In both species the Constitutive Heterochromatin (CH) rich in A-T bases is distributed in the centromeric region of all chromosomes. Eigenmannia sp. "B"... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cytogenetics; FISH; Knife fishes; Morphology; Sex chromosomes. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252015000200297 |
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| Costa,Kleber França; Brito,Rute Magalhães; Miyazawa,Carlos Suetoshi. |
| The genus Trigona contains at least 31 species, but there have been few cytogenetic studies of this group. In this work, four species of Trigona (T. branneri, T. chanchamayoensis, T. hyalinata, and T. recursa) from the municipality of Cuiabá, in the State of Mato Grosso, Brazil, were studied. In all of the species, the females had 2n = 34 chromosomes and the males had n = 17. The C-banding patterns showed that the karyotypes of these species consisted mainly of acrocentric and pseudoacrocentric chromosomes. These cytogenetic findings should useful in future phylogenetic studies of this group. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cytogenetics; Karyotype; Meliponini; Stingless bees; Trigona. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000200010 |
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| Franco,Ana Luiza; Figueredo,Amanda; Pereira,Lívia de Moraes; Sousa,Saulo Marçal de; Souza,Gustavo; Carvalho,Marcelo Ayres; Simon,Marcelo F.; Viccini,Lyderson Facio. |
| Abstract Stylosanthes (Papilionoideae, Leguminosae) is a predominantly Neotropical genus with ~48 species that include worldwide important forage species. This study presents the chromosome number and morphology of eight species of the genus Stylosanthes (S. acuminata, S. gracilis, S. grandifolia, S. guianensis, S. hippocampoides, S. pilosa, S. macrocephala, and S. ruellioides). In addition, staining with CMA and DAPI, in situ hybridization with 5S and 35S rDNA probes, and estimation of DNA content were performed. The interpretation of Stylosanthes chromosome diversification was anchored by a comparison with the sister genus Arachis and a dated molecular phylogeny based on nuclear and plastid loci. Stylosanthes species showed 2n = 20, with low... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Arachis; Cytogenetics; Evolution; Leguminosae; Stylosanthes. |
| Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000100306 |
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| Registros recuperados: 87 | |
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